Regulatory

Part:BBa_K909016:Design

Designed by: Sandro Kundert   Group: iGEM12_ETH_Zurich   (2012-09-24)

PR promoter with two operator sites: cI OR1, cI OR2 and and LacI O1/O2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This double negative hybrid promoter is only active, if cI AND lacI are absent. It functions as a biological NOR gate.

The PR region is kept conserved to prevent performance loss. The lacO1 is kept downstream of the operator similar to the natural lac operon.

Source

This (novel) design combines research of Kinkhabwala and Guet (2008) and Cox, Surette and Elowitz (2007).

References

[http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0002030 Kinkhabwala, A., & Guet, C. C. (2008) "Uncovering cis regulatory codes using synthetic promoter shuffling."]

[http://www.ncbi.nlm.nih.gov/pubmed/18004278 Cox, R. S., Surette, M. G., & Elowitz, M. B. (2007). "Programming gene expression with combinatorial promoters."]